›› 2011, Vol. 23 ›› Issue (1): 61-064.doi: 10.3969/j.issn.1004-616x.2011.01.016

• 检测研究 • Previous Articles     Next Articles

he effects of Pu'er tea on genetic damage induced by mitomycin C in human lymphoblast cell line

  

  1. 1. School of Life Sciences, Yunnan Normal University, Kunming 650092, Yunnan; 2.The Afflicated High School of Yunnan Normal University, Kunming 650118, Yunnan, China
  • Received:2010-06-17 Revised:2010-11-03 Online:2011-01-30 Published:2011-01-30
  • Contact: NI Juan

PDF (PC)

1253

Abstract: The study focused on the effects of the water extract of Pu' er tea on genetic damage induced by mitomycin C (MMC) in human lymphocyte cell line. METHODS: The lymphoblast cell line GM12593 was cultured in RPMI-1640 medium plus MMC (0.1 μg/ml) and different combinations of red and raw Pu'er tea (0~1 mg/ml) for 24 h. Cytochalasin B was added and cells were cultured for another 28 h, centrifuged and put on slides. The frequencies of micronucleated binucleated cells were analysed. RESULTS: Fermented (red) Pu'er tea (0.125 mg/ml, 0.25 mg/ml) and raw (green) Pu'er tea (0.125 mg/ml) significantly reduced the frequency of micronucleated binucleated cells induced by MMC (P<0.05). The effect of fermented Pu'er tea in preventing genetic materials induced by MMC was slightly stronger. CONCLUSION: Pu'er tea was able to reduce the genetic damage induced by MMC.

Key words: Pu' er tea, mitomycin C, micronucleus, genetic damage, human lymphoblast